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Genome Biol 12:221īackofen R, Engelhardt J, Erxleben A, Fallmann J, Grüning B, Ohler U, Rajewsky N, Stadler PF (2017) RNA-bioinformatics: tools, services and databases for the analysis of RNA-based regulation. Īxtell MJ, Westholm JO, Lai EC (2011) Vive la différence: biogenesis and evolution of microRNAs in plants and animals. Īwasthi JP, Chandra T, Mishra S, Parmar S, Shaw BP, Nilawe PD, Chauhan NK, Sahoo S, Panda SK (2019) Identification and characterization of drought responsive miRNAs in a drought tolerant upland rice cultivar KMJ 1–12-3. Īn J, Lai J, Lehman ML, Nelson CC (2012) miRDeep*: an integrated application tool for miRNA identification from RNA sequencing data. Īkhtar MM, Micolucci L, Islam MS, Olivieri F, Procopio AD (2016) Bioinformatics tools for microRNA dissection. Through this review, we discuss the recent developments and current understandings about the plant miRNAs and present the workflow of plant miRNAs including modern high-precision approaches for isolation, identification, target prediction and confirmation, miRNA-target network analysis, and functional characterization of plant miRNAs.Īfonso-Grunz F, Müller S (2015) Principles of miRNA–mRNA interactions: beyond sequence complementarity. Therefore, diverse approaches can be seen amongst the researchers which might vary in terms of specificity of analysis, such as plant-, function-, target-specific analysis of miRNAs. Current in silico progressions have made available a wide range of tools and databases for miRNA analysis.
These in silico tools are proving vital in context of identification of miRNAs, their corresponding targets, and inclusive reports on metabolic networks incorporating the identified miRNAs. A typical miRNA investigation usually incorporates preparation of wide-range small RNA library and high-throughput sequencing, followed by the computational analysis of the obtained sequences using a variety of in silico tools. Recent advancements in sequencing technologies have delivered the high-resolution clusters of sequence information from various organisms, making the miRNA characterization procedure more reliable, rapid, and promising. These small elements regulate the gene expression patterns at post-transcriptional as well as translational stages, and are proved to be concomitant with epigenetic regulations in plants during growth and developmental phases, and stress-mediated modulations/adaptations. Therefore, plant miRNAs are looked upon as one of the most potent tools for crop improvement including generation of stress resilient crops.
Plant micro-RNAs (miRNAs) are a distinct class of non-coding, small regulatory RNA molecules emerging as key regulators of growth, development, and stress responses in plants.